MétaCan
Menu
Back to cohort
Record W2020244469 · doi:10.1055/s-0028-1094073

Inhibition by Calcium of the Cyclic AMP-Mediated Stimulation of Thymic Lymphoblast Proliferation by Prostaglandin E<sub>1</sub>

2009· article· en· W2020244469 on OpenAlexaff
James Whitfield, J. MacManus, B. Braceland, Douglas J. Gillan

Bibliographic record

VenueHormone and Metabolic Research · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicEstrogen and related hormone effects
Canadian institutionsNational Research Council Canada
Fundersnot available
KeywordsStimulationInternal medicineDNA synthesisEndocrinologyCalciumLymphoblastProstaglandin E1MitosisProstaglandin ECyclic gmpChemistryCell growthCell divisionProstaglandinDNABiologyCellCell cultureCell biologyBiochemistryMedicine

Abstract

fetched live from OpenAlex

During the first 10 min after exposure of rat thymic lymphocytes (thymocytes) to a high concentration (5.0 µg/ml) of prostaglandin E 1 (PGE 1 ) in medium without added calcium, there is a 67-fold rise in the cellular cyclic AMP content which causes strong stimulations of deoxyribunucleic acid (DNA) synthesis and cell proliferation. On the other hand, in the presence of 1.0 mM calcium, PGE 1 causes a similar (68-fold) rise in the cellular cyclic AMP content and a strong stimulation of DNA synthesis, but the stimulated cells do not enter mitosis. Thus, calcium selectively inhibits the cyclic AMP-induced mitogenic process. The calcium-sensitive phase of the mitogenic reaction is normally completed by only 2.5 min after exposure to PGE 1 ; addition of calcium to the extracellular fluid at, or after, this time does not inhibit the flow of stimulated cells into mitosis which begins between 3 and 4 hr later. Finally, calcium must inhibit the operation, but not the cyclic AMP-mediated activation, of the mitogenic system since the inhibition can be fully relieved by removal of calcium at least 4 hr after exposure to PGE 1 .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.290
Teacher spread0.275 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations22
Published2009
Admission routes1
Has abstractyes

Explore more

Same venueHormone and Metabolic ResearchSame topicEstrogen and related hormone effectsFrench-language works237,207