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Record W2023410819 · doi:10.1080/07060661003740231

Relative aggressiveness and production of 3- or 15-acetyl deoxynivalenol and deoxynivalenol by<i>Fusarium graminearum</i>in spring wheat

2010· article· en· W2023410819 on OpenAlex
J. Gilbert, R. M. Clear, Todd J. Ward, Don Gaba, A. Tekauz, T. Kelly Turkington, S. M. Woods, T. W. Nowicki, Kerry O’Donnell

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
venuePublished in a venue whose home country is Canada.
aboutThe title or abstract carries a Canadian signal from the geographic lexicon.

Bibliographic record

VenueCanadian Journal of Plant Pathology · 2010
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicMycotoxins in Agriculture and Food
Canadian institutionsAlberta Crop Industry Development FundCanadian International Grains Institute
Fundersnot available
KeywordsMycotoxinFusariumInoculationBiologyVomitoxinVeterinary medicineCultivarPopulationHorticultureAgronomyZearalenoneBotanyMedicine

Abstract

fetched live from OpenAlex

Abstract Fusarium graminearum is the principal cause of fusarium head blight in North America, a disease that has caused severe losses in yield and quality of cereals. In North America, the vast majority of F. graminearum isolates produce 3- or 15-acetyl deoxynivalenol (ADON) in addition to DON. Until recently, 15-ADON isolates predominated, but a rapid shift from 15-ADON to 3-ADON producers in Canada and north central USA has been documented. In order to better understand the effect of this population shift on relative aggressiveness of isolates and mycotoxin accumulation, we tested a total of 58 isolates for 3- and 15-ADON production on two Canadian spring wheat cultivars, 'Roblin' (susceptible) and '5602 HR' (moderately resistant). In Experiment 1, three isolates from the Canadian provinces of Manitoba, Saskatchewan and Alberta, each of which produced either 15-ADON or 3-ADON, were tested using spray inoculation. In Experiment 2, 20 isolates which produced 15-ADON and 20 which produced 3-ADON from Manitoba, were tested using point inoculation. There were no significant differences in aggressiveness among isolates based either on geographic origin or mycotoxin type. Analysis of seeds from inoculated heads by gas chromatography/mass spectrometry indicated that the 3-ADON producing isolates had significantly higher DON levels than the 15-ADON isolates in 'Roblin' after both spray and point inoculation and in '5602HR' after point inoculation. DON levels following point inoculation by 15-ADON isolates were similar in the two cultivars. The 15-ADON isolates from Alberta produced less DON than 15-ADON isolates from Manitoba and Saskatchewan. Consistently, more ADON was produced by 15-ADON isolates than by 3-ADON isolates. The results of the study suggest that if the percentage of 3-ADON isolates in Canada increases, DON levels in cereals are likely to increase in epidemic years. Résumé Fusarium graminearum est la principale cause de la brûlure de l'épi en Amérique du Nord, une maladie qui engendre d'importantes pertes tant sur le plan des rendements que de la qualité chez les céréales. En Amérique du Nord, la plupart des isolats de F. graminearum produisent du 3- ou du 15-acétyl déoxynivalénol (ADON), en plus du déoxynivalénol (DON). Jusqu'à récemment, les isolats produisant du 15-ADON dominaient, mais un remplacement rapide des producteurs de 15-ADON par ceux de 3-ADON s'est opéré et a été documenté au Canada et dans le centre-nord des États-Unis. Afin de mieux comprendre ce remplacement de populations quant à l'agressivité relative des isolats et à l'accumulation de mycotoxines, nous avons testé 58 isolats relativement à la production de 3- et de 15-ADON sur deux cultivars canadiens de blé de printemps : 'Roblin' (sensible) et '5602 HR' (moyennement résistant). Au cours de l'Expérience 1, trois isolats provenant des provinces canadiennes du Manitoba, de la Saskatchewan et de l'Alberta, chacun produisant du 3- ou du 15-ADON, ont été testés par pulvérisation. Au cours de l'Expérience 2, 20 isolats qui produisaient du 15-ADON et 20, du 3-ADON provenant du Manitoba ont été testés par injection ponctuelle. Il n'y a pas eu de différence significative quant à l'agressivité chez les isolats, peu importe leur provenance ou le type de mycotoxine produit. L'analyse par couplage GC-SM des semences issues des épis inoculés a montré que les isolats produisant du 3-ADON présentaient des teneurs significativement plus élevées en DON que les isolats produisant du 15-ADON issus de 'Roblin', après avoir été inoculés par pulvérisation et injection, et dans le '5602 HR', après inoculation par injection. Les teneurs en DON qui ont fait suite aux inoculations par injection d'isolats produisant du 15-ADON étaient semblables chez les deux cultivars. Les isolats en provenance de l'Alberta, produisant du 15-ADON, ont produit moins de DON que ceux du Manitoba et de la Saskatchewan. Invariablement, de plus grandes quantités d'ADON ont été produites par les isolats 15-ADON que par les isolats 3-ADON. Les résultats de l'étude suggèrent que, si le pourcentage des isolats produisant du 3-ADON augmente au Canada, les teneurs en DON dans les céréales augmenteront probablement durant les années d'épidémies. Keywords: 3-15-acetyl deoxynivalenol3-15-ADONdeoxynivalenol Fusarium graminearum fusarium head blightmycotoxin Triticum aestivum Mots clés: 3-15-acétyl déoxynivalénol3-15-ADONbrûlure de l'épidéoxynivalénol Fusarium graminearum mycotoxine Triticum aestivum Acknowledgements The authors gratefully acknowledge Ron Kaethler, Kirsten Slusarenko and Courtney Leclerc for technical support and the Western Grains Research Foundation and Matching Investments Initiative for financial support.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.813
Threshold uncertainty score0.968

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.188
Teacher spread0.178 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it