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Record W2023464325 · doi:10.1080/07924259.2000.9652396

Structure and function of the posterior vas deferens of the snow crab, <i>Chionoecetes opilio</i> (Brachyura, Majidae)

2000· article· en· W2023464325 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueInvertebrate Reproduction & Development · 2000
Typearticle
Languageen
FieldEnvironmental Science
TopicCrustacean biology and ecology
Canadian institutionsFisheries and Oceans Canada
Fundersnot available
KeywordsSpermatophoreEndoplasmic reticulumGolgi apparatusVas deferensBiologyCytoplasmLumen (anatomy)AnatomyUltrastructureCell biologySperm

Abstract

fetched live from OpenAlex

Summary The structure and function of the posterior vas deferens (PVD) of the male snow crab, Chionoecetes opilio, were studied by light and electron (SEM/TEM) microscopy. The vas deferens is divided into three regions: anterior (AVD), median (MVD) and posterior (PVD). Spermatophores are formed in the AVD and moved to the MVD where they are stored within droplets of seminal fluid. Seminal fluid and spermatophores are strongly PAS positive. The reaction with alcian blue-PAS indicated the presence of neutral polysaccharides. The PVD is further divided into the distal and proximal regions that differ in structure and function. In the distal region of the PVD, the epithelial cells are columnar containing lobed nuclei. The lumen contains a mixture of lysed spermatozoa and spermatophores. Numerous heterophagic vacuoles present at the cell surface are absorbed and digested intracellularly. The cytoplasm contains a large amount of rough endoplasmic reticulum and lysosomes associated with Golgi complexes and few mitochondria. TEM observations reveal that the primary function of this region is the phagocytosis of excess spermatozoa. The digestive function of the distal region of the PVD suggests that this mode of secretion is apocrine. The proximal region of the PVD contains neither spermatophores nor free spermatozoa. The epithelium is composed of cuboidal cells with elongate nuclei. The apical microvilli are short and regularly arranged. The lumen contains a large quantity of an amorphous, jelly-like substance, which is the secretory product of the distal region of the PVD. In the cytoplasm rough endoplasmic reticulum and Golgi complexes are absent. We conclude that the proximal region of the PVD, therefore, is used for storage of highly acidic seminal fluid. The function of the PVD for the destruction and resorption of spermatophores and spermatozoa has not previously been reported in other decapods. Key words: Vas deferensultrastructurephagocytosiscrustacean Chionoecetes

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.090
Threshold uncertainty score0.997

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0040.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.184
Teacher spread0.176 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it