Phospholipase C Involvement in Activation of the Muscarinic Receptor-Operated Cationic Current in Guinea Pig Ileal Smooth Muscle Cells
Bibliographic record
Abstract
In guinea pig single ileal smooth muscle cells held under voltage-clamp, the role of phospholipase C (PLC) in activation of the muscarinic receptor-operated cationic current (I(cat)) was studied. U73122, a PLC inhibitor, prevented the generation of I(cat) by the muscarinic agonist carbachol. The effect did not involve muscarinic receptor block since it also blocked I(cat) which was evoked by GTPgammaS applied intracellularly to activate G proteins bypassing muscarinic receptors. Also, neither cationic channel block nor other possible nonspecific actions seemed to be involved since its analogue (U73343), structurally close but deficient of the PLC-inhibiting activity, did not significantly affect carbachol- or GTPgammaS-evoked I(cat). Antibodies against the alpha subunits of G(q)/G(11) proteins (Galpha(q)/Galpha(11)-antibody) blocked only the small component of carbachol-evoked I(cat), which was associated with an increase in [Ca(2+)](i) linked to an increase in G(q/11) protein-regulated PLC activity. 1-Oleoyl-2-acetyl-sn-glycerol (OAG), an analogue of diacylglycerol (DAG) produced via PLC-catalyzed metabolism, produced no or only a small current by itself, with the carbachol-evoked I(cat) remaining unchanged. These results provide evidence for the importance of PLC in I(cat) generation, and they also strongly suggest that the activity of PLC involved in the primary activation of I(cat) is neither under regulation by G(q/11) proteins nor dependent on the action of DAG.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".