156 THE USE OF THE DG29™ ENZYME-LINKED IMMUNOSORBENT ASSAY KIT TO PREDICT PREGNANCY PRIOR TO EMBRYO TRANSFER IN LACTATING HOLSTEIN COWS
Bibliographic record
Abstract
Evaluation of postpartum fertility in cows is important for the efficient management of reproduction. DG29™ enzyme-linked immunosorbent assay (ELISA) kit (Conception, Animal Reproduction Technologies, Canada) measures the level of pregnancy–related glycoproteins in blood that are linked to pregnancy in the bovine species. The proteins are known to persist in the postpartum period. Here, we investigated whether the postpartum fertility in Holstein dairy cows can be evaluated through the use of the DG29 kit. We confirmed that genital organs of lactating Holstein cows (n = 119, from Days 56 to 688 postpartum) were normal by a 5.0/7.5-MHz linear array transducer (Tringa, Pie Medical Equipment B.V., Maastricht, The Netherlands), then a progesterone releasing intravaginal device (PRID; CEVA Sante Animale, Libourne, France) was inserted (Day 0) and maintained for 9 days. On Day 7, PGF2a was administered (2 mL Dalmazine, Kyoritsu Seiyaku, Tokyo, Japan). Blood samples were collected from the tail vein or artery into vacuum tubes at the time of PRID insertion. Serum was separated and stored at –30°C until the ELISA was performed. Oestrus (Day 0) was detected by visual observation. Fresh embryos recovered from Japanese Black cows were transferred to 119 recipient cows in various parities (primiparous = 70, biparous = 27, and multiparous = 22) on Days 6 to 8 of oestrous cycle. Pregnancy was diagnosed between Days 40 to 60 by transrectal ultrasonography. The statistical significance of any differences between various parities was assessed by chi-squared and Student’s t-tests. The pregnancy rate was higher for primiparous cows than for biparous and multiparous cows (64.3, 55.6, and 54.5%, respectively), while concentrations of the pregnancy-related glycoproteins in primiparous cows (135.0 ± 29.8 pg mL–1) were significantly lower than those of biparous (389.4 ± 175.9 pg mL–1) and multiparous cows (399.2 ± 203.1 pg mL–1, mean ± SEM; P < 0.05). In primiparous and multiparous cows, the concentrations of pregnancy-related glycoproteins were significantly lower in pregnant cows compared with nonpregnant cows (primiparous: 81.1 ± 29.9 v. 232.6 ± 59.8 pg mL–1; P < 0.05; multiparous: 20.8 ± 16.2 v. 853.4 ± 411.5 pg mL–1; P < 0.05). However, there was no significant difference between pregnant and nonpregnant biparous cows. In conclusion, the DG29 kit may be useful for the prediction of postpartum fertility in lactating Holstein cows. Further studies are needed to test the validity of this observation by using a greater number of various parties’ cows.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".