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Record W2029009493 · doi:10.1159/000132056

Ultrastructural organization of double minute chromosomes and HSR regions in human colon carcinoma cells

2008· article· en· W2029009493 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueCytogenetics and Cell Genetics · 2008
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicDNA and Nucleic Acid Chemistry
Canadian institutionsUniversity of Calgary
Fundersnot available
KeywordsKaryotypeBiologyChromosomeUltrastructureCentromereMolecular biologyChromosome 22Marker chromosomeKinetochoreGeneticsAnatomyGene

Abstract

fetched live from OpenAlex

The ultrastructural organization of double minute chromosomes (DMs) and marker chromosomes displaying homogeneously staining regions (HSRs) have been investigated in two cell lines, COLO-320 DM and COLO-320 HSR, derived from a human colon carcinoma. Double minute chromosomes in the COLO-320 DM cell line vary in number from several to several hundred and in diameter from 0.5 to 2 micron. These chromosomes lack kinetochore structures which appear as discrete electron dense plates on the centromeric chromosomes. The DMs are composed of regularly arranged nucleosome-containing fibers. These are folded to form 30 nm fibers which extend as loops from the central portion of the chromosome. In COLO-320 HSR, a majority of cells contain a single submetacentric HSR marker chromosome with HSR regions in both chromosome arms. The HSR chromosome appears structurally analogous in EM preparations to the other centromeric chromosomes of the karyotype.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.095
Threshold uncertainty score0.780

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.206
Teacher spread0.196 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it