Fluctuations of γ-aminobutyrate, γ-hydroxybutyrate, and related amino acids in <i>Arabidopsis</i> leaves as a function of the light–dark cycle, leaf age, and N stressEditorial decisions for this paper were made by Robert Ireland, Associate Editor, <i>Canadian Journal of Botany</i>.
Why this work is in the frame
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Bibliographic record
Abstract
To gain further insight into the metabolic role of γ-aminobutyrate (GABA), we determined the pool sizes of GABA and its catabolic products, alanine and γ-hydroxybutyrate (GHB), as well as key amino acids (Glu, Gln, Asp, Asn, Pro, Gly, Ser), in Arabidopsis leaves as a function of the light–dark cycle, leaf age (old versus young), and N stress (continuous versus interrupted N supply). Regardless of time of day and leaf age, there was a close relationship among Glu, GABA, and GHB when N was supplied continuously, indicating that GABA and GHB were probably derived exclusively from Glu and GABA, respectively. Ala was also closely linked to GABA in young leaves, but not in old leaves, a result consistent with the existence of multiple sources of Ala. The nature of the responses of GABA and GHB to an interrupted N supply depended on leaf age, and differed from responses exhibited by Glu, Gln, and Asn. Overall fluctuations in primary amino acids under both continuous and interrupted N supply, as well as those associated with photorespiration, aging, and stress, suggest that the old and young leaves chosen for study here function in Arabidopsis as source and sink leaves, respectively.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it