Transcription and Expression of Major VP Gene of Bombyx Mori Parvo-like Virus
Bibliographic record
Abstract
The Bombyx mori parvo-like virus (China Zhenjiang isolate), termed as BmDNV-Z, replicates only in host’s midgutcolumnar cells and causes flacherie disease. The viral genome is composed of two sets of different single-strandedlinear DNA molecules, Viral DNA 1(VD1) and Viral DNA 2(VD2). Here we cloned the major VP gene from theplasmid contained viral DNA fragments. The gene consists of 1500 nucleotides and the deduced protein has 499 aminoacid residues, with the predicted molecular weight of 54934.24Da, isoelectric point of 6.73. The coding sequence wasinserted into expression plasmid vector pET-30a and expressed in Escherichia coli BL21 (DE3) under the induciblepromoter LacZ. The expressed product was detected by Western blotting with anti-His antibody. Interestingly, there arefour laddered band instead of one. We speculated that this virus may use leaky scanning mechanism to express fourproteins in one ORF. And we investigated the major VP gene transcriptional time phase in susceptible silkworm throughreal time quantitative PCR (qPCR). The result showed that the mRNA level of VP gene increased greatly after 24 hourspost inoculation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".