Prevalence of Puroindoline Grain Hardness Genotypes among Historically Significant North American Spring and Winter Wheats
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Grain hardness (“hard” or “soft” kernel texture) is the single most important trait in determining the utilization and marketing of wheat ( Triticum aestivum L.). Puroindoline a and b proteins represent the molecular basis for this trait. This study surveyed the prevalence of puroindoline hardness mutations (alleles) among North American spring and winter wheat varieties with emphasis on those that are historically important. Each variety was assessed for kernel texture using the Single Kernel Characterization System; Hardness alleles were defined by puroindoline gene sequence and the presence or absence of puroindoline a protein on polyacrylamide gels. A total of 90 spring wheats were examined: nine were soft and possessed wild‐type (“soft”) puroindoline sequences, 10 were mixed hardness, and the remaining 71 were uniformly hard. Of these hard spring wheats, 18 carried the Pina‐D1b hardness allele (null for puroindoline a protein), 47 the Pinb‐D1b allele (Gly‐46–Ser‐46), and four the Pinb‐D1c allele (Leu‐60–Pro‐60). Two hard spring wheats possessed a new allele, designated Pinb‐D1e , which involves a single nucleotide change in Trp‐39 to a “stop” codon. Lastly, among the spring wheats, a new hardness allele was found in the hard component of the variety ‘Utac’ which was mixed. This allele, Pinb‐D1f , also involved a single nucleotide change such that Trp‐44 became a “stop” codon. A total of 62 winter wheat varieties were examined, of which five were soft and three were of mixed hardness. Of interest, the three mixed hardness wheats were ‘Turkey’, ‘Kharkof’, and ‘Weston’. The hard component of each carried the Pinb‐D1b allele. Of the 54 remaining wheats, all of which were hard, all but two carried this same Pinb‐D1b allele. ‘Chiefkan’ winter wheat carried the same Pinb‐D1e allele as ‘Canadian Red’ and ‘Gehun’ spring wheats. ‘Andrews’ hard red winter wheat possessed a new allele, designated Pinb‐D1g , which was a single nucleotide change in Cys‐56 to a “stop” codon. In conclusion, hard grain phenotype results from one of various mutations in either of the puroindoline proteins. To‐date, seven hardness alleles have been discovered and characterized in hexapoid wheat. All but one occur in the puroindoline b gene coding sequence and result from single nucleotide changes. These molecular markers are useful in characterizing lineages and analyzing ancestral relationships.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it