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Record W2030990049 · doi:10.1080/07060660709507480

Diversity of <i>Fusarium</i> species associated with discolored ginseng roots in British Columbia

2007· article· en· W2030990049 on OpenAlexfundvenueaboutno aff
Zamir K. Punja, A. Wan, Rubella S. Goswami, Neha Verma, Mahfuzur Rahman, Tharcisse Barasubiye, Keith A. Seifert, C. André Lévesque

Bibliographic record

VenueCanadian Journal of Plant Pathology · 2007
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant Pathogens and Fungal Diseases
Canadian institutionsnot available
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsGinsengFusariumBiologyInoculationRoot rotBotanyAmerican ginsengPathogenicityHorticultureMicrobiology

Abstract

fetched live from OpenAlex

Crown discoloration on roots of American ginseng (Panax quinquefolius) is a major problem that causes a reduction in quality and can be a limiting factor to ginseng production in some regions of Canada. The symptoms result from an accumulation of phenolic compounds within disorganized and disrupted cells, accompanied by the presence of fungal hyphae in affected cells. Twelve Fusarium species were recovered on isolation media during June-September of 2004 and 2005 from ginseng roots displaying root-surface discoloration in British Columbia. Many of the same species were also recovered from soil from ginseng fields and cereal-straw mulch used during ginseng production. Hybridization of labeled PCR amplicons from DNA samples from affected root tissue to a DNA array designed using oligonucleotides specific to 26 Fusarium species was used to identify the Fusarium species present in ginseng roots. Over 90% of symptomatic root samples contained F. equiseti and several other Fusarium species, including F. sporotrichioides and F. avenaceum, that were absent in nonsymptomatic tissues. An in vitro pathogenicity test was used to screen 180 Fusarium isolates originating from affected roots. Reddish brown lesions developed 10 days following inoculation with 58 isolates of F. equiseti and F. sporotrichioides and to a lesser extent with 7 isolates of F. avenaceum and F. culmorum. Greenhouse and field inoculations confirmed the pathogenicity of F. equiseti and F. sporotrichioides isolates to ginseng roots. Pathogenicity tests conducted with 340 additional isolates representing 10 Fusarium species originating from wheat- and barley-straw mulch and soil from ginseng fields, as well as from cereal hosts worldwide, identified a proportion of isolates (52.5%) of F. equiseti, F. sporotrichioides, F. avenaceum, and F. culmorum that produced reddish brown lesioned areas. These cereal-infecting Fusarium species can induce a tissue response following infection that results in root-surface discoloration. Key words: epidemiology, molecular detection, Panax quinquefolius, rusty root. La coloration anormale des racines de ginseng (Panax quinquefolius) est un problème important qui diminue la qualité et peut être un facteur limitant pour la production de ginseng dans certaines régions du Canada. Les symptômes sont le résultat d'une accumulation de composés phénoliques à l'intérieur de cellules désorganisées et désintégrées, accompagnée de la présence d'hyphes fongiques dans les cellules affectées. Entre juin et septembre de 2004 et de 2005, en Colombie-Britannique, douze espèces de Fusarium furent obtenues sur milieu de culture à partir de racines de ginseng montrant, en surface, des symptômes de coloration anormale. Les mêmes espèces furent aussi isolées du sol de culture de ginseng et de paillis de céréales utilizé pour la production du ginseng. Pour identifier les espèces de Fusarium présentes dans les racines de ginseng, l'hybridation d'amplicons marqués obtenus par PCR à partir de l'ADN d'échantillons de tissu racinaire affecté fut utilizée sur puces à ADN avec oligonucléotides spécifiques aux 26 espèces de Fusarium. Plus de 90 % des racines avec symptômes contenaient le F. equiseti et plusieurs autres espèces de Fusarium, y compris le F. sporotrichioides et le F. avenaceum, qui étaient absents des tissus sans symptômes. Un test de pathogénicité in vitro fut fait pour trier 180 isolats de Fusarium provenant de racines affectées. Des lésions brun rougeâtre se sont développées 10 jours après l'inoculation de 58 isolats de F. equiseti et de F. sporotrichioides, et, à un moindre degré, de 7 isolats de F. avenaceum et de F. culmorum. Des inoculations en serre et au champ ont confirmé le pouvoir pathogène des isolats de F. equiseti et de F. sporotrichioides sur des racines ginseng. Les tests de pathogénicité, menés avec 340 isolats additionnels représentant 10 espèces de Fusarium provenant de paillis de blé et d'orge et de sol de champs de ginseng, ainsi que de céréales à travers le monde, ont permis de déterminer le pourcentage d'isolats (52,5 %) de F. equiseti, de F. sporotrichioides, de F. avenaceum et de F. culmorum qui produisent des plages de lésions brun rougeâtre. Ces espèces de Fusarium des céréales peuvent induire une réponse du tissu après infection suivie par l'apparition de coloration anormale de la surface des racines. Mots-clés : épidémiologie, détection moléculaire, Panax quinquefolius, racine rouille.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.922
Threshold uncertainty score0.997

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.174
Teacher spread0.166 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations35
Published2007
Admission routes3
Has abstractyes

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