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RFLP analysis of Hordeum species relationships1

2008· article· en· W2032810523 on OpenAlexaff
Stephen J. Molnar, Roger Wheatcroft, George Fedak

Bibliographic record

VenueHereditas · 2008
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Disease Resistance and Genetics
Canadian institutionsAgriculture and Agri-Food Canada
Fundersnot available
KeywordsBiologyHordeum vulgareEcoRIXhoIRestriction fragment length polymorphismRibosomal DNARestriction enzymeHindIIIHordeumSouthern blotGeneticsRibosomal RNARestriction siteBotanyBamHIRestriction mapPhylogenetic treePoaceaeDNANucleic acid sequenceGenePolymerase chain reaction

Abstract

fetched live from OpenAlex

Detailed ribosomal DNA restriction maps were prepared for eight representative plants from Hordeum L. Section Hordeum (two H. bulbosum, H. glaucum, a diploid H. leporinum, a tetraploid H. leporinum, H. murinum, H. spontaneum, H. vulgare) in order to explore species relationships. The four restriction enzymes (EcoRI,SacI, BamHI and HindIII) used in our earlier study were supplemented with six additional ones (BglI, ClaI, KpnI, Pvu II, Sst II and XhoI). Southern blots prepared, using the restriction enzymes singly and in combinations, were probed with the wheat ribosomal probe pTA7l, and hybridization patterns analyzed. A comparison of the derived maps with known maps for wheat, pea and pumpkin showed strong conservation of restriction sites, especially in the ribosomal coding region. The maps suggest that duplication of coding region DNA and its insertion into the spacer region as well as amplification of subrepeats are responsible for variation in rDNA repeat length. Phylogenetic analysis indicated the early divergence of ancestral wheat and Hordeum species, followed by the divergence of the progenitors of H. spontaneum/H. vulgare and the remaining Hordeum species and, most recently, between those of H. glaucum and H. murinum.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.101
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.204
Teacher spread0.158 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations19
Published2008
Admission routes1
Has abstractyes

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