212 EFFECT OF A LOW DOSE OF eCG ON SUPEROVULATION AND EMBRYO COLLECTION IN WOOD BISON DURING THE BREEDING SEASON
Bibliographic record
Abstract
In an effort to conserve a threatened Canadian species, Bison bison athabascae, we developed an embryo collection protocol subsequent to superovulatory treatment involving two doses of FSH diluted in hyaluronan given 48 h apart. The follicular response to superstimulatory treatment was satisfactory, but many follicles did not ovulate, thus limiting the number of embryos collected. Based on recent results in cattle, where replacement of the final doses of FSH with a low dose of eCG resulted in the recovery of a greater number of ova/embryos, the objective of the present study was to evaluate the effect of adding eCG to the superovulatory protocol to increase ovulation rate, embryo collection, and embryo quality in wood bison during the breeding season (September). Ovarian synchronization was induced in wood bison (n = 24) by treatment with a luteolytic dose of prostaglandin (500 mcg, Cloprostenol) followed 8 days later by transvaginal ultrasound-guided follicular ablation. Follicular wave emergence (Day 0) was defined as the day after follicle ablation. Bison were assigned randomly to two groups: FSH (n = 12) and FSH+eCG (n = 12). FSH was diluted in hyaluronan (5 mg mL–1, MAP-5, Bioniche Animal Health, Belleville, ON, Canada) and given intramuscularly on Day 0 (300 mg) and Day 2 (100 mg) in both groups. Bison in the FSH+eCG group received 450 IU eCG (Pregnecol, Bioniche Animal Health) intramuscularly on Day 3, and bison in both groups were administered a luteolytic dose of prostaglandin on Day 3. On Day 5, bison were given 2500 IU hCG (Chorulon, Merck Animal Health, Summit, NJ, USA) intramuscularly to induce ovulation. The bison were artificially inseminated with chilled semen 12 and 24 h after hCG treatment. Nonsurgical embryo collection was performed on Day 13. The ovaries were examined by transrectal ultrasonography on Days 5, 7, and 13 to record the follicular response, ovulation rate, and number of corpora lutea (CL), respectively. Results were compared between groups by t-test or chi-square test (Table 1). The number of ovulatory-sized follicles (=9 mm), ovulation rate (number ovulations/ovulatory-sized follicles), number of CL, number of ova/embryos, and number of transferable embryos were not different between groups (P = 0.23, P = 0.19, P = 0.25, P = 0.18, P = 0.09, respectively). In conclusion, the superovulatory response and embryo collection rate in wood bison approached that observed in cattle, but were not improved by the addition of a low dose of eCG. Table 1. Response of wood bison (mean ± s.e.m.) to superovulatory treatment with or without eCG
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".