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Record W2033391507 · doi:10.1159/000242003

Effects of Dexamethasone Administration to Pregnant Rats on Adenylate Cyclase and Cyclic AMP Phosphodiesterase Activities in the Maternal and Fetal Lungs

2009· article· en· W2033391507 on OpenAlexaff
K. C. Chaudhary, M.S. Nijjar

Bibliographic record

VenueBiology of the Neonate · 2009
Typearticle
Languageen
FieldMedicine
TopicNeonatal Respiratory Health Research
Canadian institutionsUniversity of Manitoba
FundersMedical Research Council
KeywordsCyclaseInternal medicineEndocrinologyAdenylate kinasePhosphodiesteraseActivator (genetics)DexamethasoneFetusPhosphodiesterase inhibitorBiologyEnzymeMedicineStimulationPregnancyBiochemistryReceptor

Abstract

fetched live from OpenAlex

Adenylate cyclase and cyclic AMP phosphodiesterase activities were examined in the maternal and fetal lungs before and after delivery from the saline- and dexamethasone-treated pregnant rats. Basal adenylate cyclase activity in the maternal lungs of control animals did not change after parturition whereas the enzyme activity in fetal lungs increased 2.5-fold after birth. Dexamethasone treatment reduced adenylate cyclase activity in the maternal lungs before and after parturition while the enzyme activity in fetal lungs was increased. The ability of the cytoplasmic factors to activate adenylate cyclase in maternal lungs decreased after parturition. Dexamethasone treatment of the pregnant rat lowered the cytoplasmic activation of the maternal enzyme before and after parturition, but the percent activation of the enzyme in both the maternal and fetal lungs was not affected. The decrease in activator activity of the maternal lungs after parturition was not apparently due to the reduced sensitivity of adenylate cyclase to the same level of the activator protein. However, it would appear that both adenylate cyclase and its activator protein have undergone some maturational changes during lung development. Adrenalectomy of rats did not alter the basal adenylate cyclase activity in lung membranes but reduced the cytoplasmic activation of the enzyme. Unlike pregnant rats, dexamethasone treatment of adrenalectomized rats restored the cytoplasmic activation of adenylate cyclase not only to the control value, but potentiated it even higher than the control without affecting the basal adenylate cyclase activity. Cyclic AMP phosphodiesterase activity in both the maternal and fetal lungs was not influenced by parturition. However, dexamethasone treatment lowered cyclic AMP phosphodiesterase activity in both the maternal and fetal lungs at both ages. Dexamethasone treatment of pregnant rats elevated phospholipids and lowered DNA levels in the maternal as well as fetal lungs. These data are consistent with the view that dexamethasone treatment of pregnant rats during late gestation may elevate cyclic AMP levels to induce fetal lung maturation with a concomitant decrease in cellular proliferation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.422
Threshold uncertainty score0.271

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.345
Teacher spread0.322 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations8
Published2009
Admission routes1
Has abstractyes

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