Optimized production of a safe and efficient gene therapeutic vaccine versus HIV via a linear covalently closed DNA minivector
Bibliographic record
Abstract
Conventional plasmid vectors (pDNA) possess unwanted prokaryotic DNA that when delivered to the mammalian cells can reduce transgene expression and integrate into the human genome, potentiating oncogenesis. Bacterial sequence-free, linear covalently closed (LCC) DNA minivectors have shown improved safety and efficacy both in vitro and in vivo. We previously constructed and characterized an in vivo LCC DNA minivector (ministring) system that can convert pDNA into DNA ministrings. Here we sought to optimize this system and apply ministrings to encode a gene to generate HIV virus-like particles (VLPs) in targeted cells. The DNA-VLP expresses gagV3(BCE) gene in target cells to simultaneously yield GagV3(BCE) VLP assembly and presentation of immunodominant HIV antigens, eliciting production of neutralizing antibodies and cytotoxic T-cell responses against HIV.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".