A Novel Lensless Miniature Contact Imaging System for Monitoring Calcium Changes in Live Neurons
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Bibliographic record
Abstract
Here, we report on the design, fabrication, and verification of a novel CMOS-imager-based contact imaging system. We acquired fluorescent images from live neurons by monitoring calcium changes with Fura-2 dye. Our current device consists of a removable absorption filter interfaced with a CMOS imaging sensor and an external DG-4 lamp for excitation. Fura-2 loaded Lymnaea stagnalis neurons were stimulated with dual excitation wavelengths of 340 and 380 nm; our image sensor detected 510-nm emission. We show that our system is capable of detecting intracellular calcium changes in Fura-2 loaded neurons. Further, this sensor also enabled viewing of multiple neurons over a large surface area simultaneously, an option that is not readily available in conventional light microscopy.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it