Protein Kinase A(PKA)-Restrictcive and PKA-Permissive Phases of Oocyte Maturation
Bibliographic record
Abstract
Substantial evidence has indicated that cAMP-dependent protein kinase (protein kinase A or PKA) plays a critical role in maintaining meiotic prophase arrest in vertebrate oocytes. However, PKA activity dynamic and its physiological substrate profile during oocyte maturation remain poorly defined. We have recently developed a novel PKA substrate construct which we employ to monitor PKA activity in live oocytes. We demonstrated here that, during progesterone-induced oocyte maturation, PKA was inactivated within 30 min of the addition of progesterone and thereafter remained inactivated throughout the entire maturation process. However, artificial reactivation of endogenous PKA had differential consequences, depending on the timing of PKA reactivation. Reactivation of endogenous PKA at any time prior to germinal vesicle breakdown (GVBD) inhibited progesterone-induced GVBD. PKA reactivation at GVBD, or thereafter, did not interfere with meiosis I to meiosis II transition, nor did it interfere with metaphase II arrest. These results demonstrate for the first time a PKA-restrictive and a PKA-permissive phase in oocyte maturation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".