Protein Kinase A(PKA)-Restrictcive and PKA-Permissive Phases of Oocyte Maturation
Bibliographic record
Abstract
Substantial evidence has indicated that cAMP-dependent protein kinase (protein kinase A or PKA) plays a critical role in maintaining meiotic prophase arrest in vertebrate oocytes. However, PKA activity dynamic and its physiological substrate profile during oocyte maturation remain poorly defined. We have recently developed a novel PKA substrate construct which we employ to monitor PKA activity in live oocytes. We demonstrated here that, during progesterone-induced oocyte maturation, PKA was inactivated within 30 min of the addition of progesterone and thereafter remained inactivated throughout the entire maturation process. However, artificial reactivation of endogenous PKA had differential consequences, depending on the timing of PKA reactivation. Reactivation of endogenous PKA at any time prior to germinal vesicle breakdown (GVBD) inhibited progesterone-induced GVBD. PKA reactivation at GVBD, or thereafter, did not interfere with meiosis I to meiosis II transition, nor did it interfere with metaphase II arrest. These results demonstrate for the first time a PKA-restrictive and a PKA-permissive phase in oocyte maturation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".