Immunolocalization of Tomato Mosaic Tobamovirus in Roots of Red Spruce Seedlings
Bibliographic record
Abstract
Abstract The objective of this study was to determine the location of tomato mosaic tobamovirus (ToMV) in red spruce (Picea rubens) seedling roots. Roots were collected from uninfected and infected spruce seedlings at biweekly intervals, fixed in formaldehyde‐glacial acetic acid‐50% ethanol (1 : 1 : 18), embedded in paraffin, sectioned, and tested by indirect immunofluorescence (IF). ToMV was detected by IF in infected, but not in uninfected red spruce roots. All seedlings that tested positive by IF had previously tested positive by enzyme‐linked immunosorbent assay. Fluorescence was observed most frequently 2 weeks after inoculation in cortical and epidermal cells, as well as in the lateral root primordia. The ToMV was not observed in vascular tissue, the root cap, or meristematic cells. Virus distribution was uneven, and occurred in localized, concatenated pockets, parallel to the axis of the apical meristem. In older seedlings, fluorescence was associated with irregular‐shaped vesicles in cortical cells.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".