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Immunolocalization of Tomato Mosaic Tobamovirus in Roots of Red Spruce Seedlings

2001· article· en· W2046215581 on OpenAlex
George D. Bachand, John D. Castello

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

fundA Canadian funder is recorded on the work.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueJournal of Phytopathology · 2001
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Virus Research Studies
Canadian institutionsnot available
FundersMcMaster University
KeywordsBiologyTobamovirusMeristemSeedlingBotanyPrimordiumInoculationFibrous root systemPlant virusHorticultureVirusVirologyShoot

Abstract

fetched live from OpenAlex

Abstract The objective of this study was to determine the location of tomato mosaic tobamovirus (ToMV) in red spruce ( Picea rubens ) seedling roots. Roots were collected from uninfected and infected spruce seedlings at biweekly intervals, fixed in formaldehyde‐glacial acetic acid‐50% ethanol (1 : 1 : 18), embedded in paraffin, sectioned, and tested by indirect immunofluorescence (IF). ToMV was detected by IF in infected, but not in uninfected red spruce roots. All seedlings that tested positive by IF had previously tested positive by enzyme‐linked immunosorbent assay. Fluorescence was observed most frequently 2 weeks after inoculation in cortical and epidermal cells, as well as in the lateral root primordia. The ToMV was not observed in vascular tissue, the root cap, or meristematic cells. Virus distribution was uneven, and occurred in localized, concatenated pockets, parallel to the axis of the apical meristem. In older seedlings, fluorescence was associated with irregular‐shaped vesicles in cortical cells.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.834
Threshold uncertainty score0.132

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.262
Teacher spread0.240 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it