Osteopontin Modulates Malignant Pleural Mesothelioma Cell Functions in Vitro
Bibliographic record
Abstract
目的．オステオポンチン（OPN）はインテグリンαvβ3を介して癌の進展や血管新生などに関与する接着分子であり，アスベストに曝露された悪性胸膜中皮腫患者の血清マーカーとして有用であることが報告された．本研究では悪性胸膜中皮腫細胞株におけるOPNの発現，接着，増殖，アポトーシスにおける役割について検討する．方法．各種の中皮腫細胞株を用いOPNの発現，細胞表面上の各種インテグリンの発現を評価した．固相化したOPNと細胞株との接着，増殖試験を抗αvβ3抗体もしくはArginine-Glycine-Aspartic acid（RGD）ペプチドの添加·非添加の条件下で行った．同様にOPN上でのアポトーシスと遊走能の評価と固相化OPN上におけるfocal adhesion kinase（FAK）のリン酸化を検討した．結果．Reverse transcriptase-polymerase chain reaction（RT-PCR）の結果，肉腫型胸膜中皮腫細胞株でOPNの発現を認めた．同様にFACScanTMではαvとβ3の発現とOPNに対しての接着，増殖の増強を認めたが，抗αvβ3抗体もしくはRGDペプチドの添加により阻害された．またOPN上で抗アポトーシス作用と，FAKのリン酸化の増強が認められた．結論．肉腫型中皮腫細胞株においてOPNに対する接着，増殖の増強，抗アポトーシス，FAKのリン酸化の増強が認められた．この機序にインテグリンαvβ3を介するシグナルが寄与している可能性が示唆された．
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".