Effect of dietary Ca manipulation, anionic salts and supplemental vitamin D<sub>3</sub> on calcium homeostasis of finishing steers
Bibliographic record
Abstract
The objective of this study was to evaluate the effects of a low-Ca diet, followed by a high-Ca diet in combination with feeding of anionic salts and/or vitamin D3 on Ca and acid-base homeostasis of finishing steers. Twenty Hereford steers (448 _ 26 kg) were individually penned and fed at 0800 and 1600 with a ration consisting of 90% barley grain-based concentrate and 10% barley silage (DM basis). The experimental protocol included a 19-d adaptation period, a 14-d period of low Ca feeding, a 10-d supplemental period and a 5-d withdrawal period. During the supplemental period, anionic salts (MgSO4 and NH4Cl) were incorporated into the diet at -1500 and -3000 mEq steer-1 d-1 for 3 and 7 d, respectively. The cattle were also supplemented with one of four vitamin D3 treatments: 0, 0.6, 1.2 and 2.4 million IU (MIU) of vitamin D3 steer-1 d-1. Blood samples were obtained for measurement of total and ionized Ca, parathyroid hormone (PTH) and calcitonin, vitamin D3, 25(OH)D3 and 1,25(OH)2D3. Feed intake was depressed (P < 0.05) by anionic salt feeding but not vitamin D3 treatment. Cattle supplemented with vitamin D3 had higher total (P < 0.05) and ionized (P < 0.05) serum Ca levels than the control cattle that were only supplemented with anionic salts. Higher (P < 0.04) ionized Ca levels were seen in cattle fed 2.4 versus those fed 0.6 MIU vitamin D3. Maximum total serum Ca values were 10.11, 10.97, 11.43 and 12.24 mg dL-1 for the 0, 0.6, 1.2 and 2.4 MIU vitamin D3 treatments, respectively. Respective maximum values for ionized Ca were 5.43, 5.90, 5.98 and 6.25 mg dL-1. These values represent increases, relative to the adaptation period of 3, 12, 16 and 25% for total and 9, 18, 20 and 31% for ionized Ca for the 0, 0.6, 1.2 and 2.4 MIU D3 treatments, respectively. Circulating concentrations of vitamin D3, 25(OH)D3, and 1,25(OH)2D3 were increased (P < 0.05) by vitamin D3 feeding, however, PTH was decreased (P < 0.05), while calcitonin was not affected (P > 0.05). Anionic salt feeding induced (P < 0.05) a mild systemic acidosis. The results indicate that anionic salt feeding enhanced the response of serum Ca to vitamin D3 supplementation. Higher serum Ca levels prior to slaughter may increase intra-muscular Ca levels and enhance postmortem activity of Ca-dependant proteases responsible for myofibril degradation. The results point to a dietary strategy that with further research may prove useful in enhancing beef tenderness. Of particular interest is that serum Ca levels were elevated when vitamin D3 was supplemented at levels 25 to 50% or less than levels used in previous research. Key words: Calcium homeostasis, vitamin D3, anionic salts, beef tenderness
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".