First Report of Canola Blackleg Caused by Pathogenicity Group 4 of <i>Leptosphaeria maculans</i> in Manitoba
Bibliographic record
Abstract
Leptosphaeria maculans (Desmaz.) Ces. & de Not., causal agent of blackleg of canola (Brassica napus L.), was initially placed in several pathogenicity groups (PG) on the basis of the interaction phenotypes (IP) of L. maculans isolates on the differential canola cvs. Westar (W), Glacier (G), and Quinta (Q) (4). PG1 isolates are weakly virulent and PG2, PG3, and PG4 isolates are highly virulent. In Manitoba, the L. maculans population consists mainly of PG2 isolates (virulent on W and avirulent on G and Q), a few PG1 isolates (avirulent on W, G, and Q), and PGT (virulent on W and Q, but avirulent on G) (3). Since the blackleg fungus is known to have a high level of evolutionary potential, the Oilseed Pathology Laboratory at the University of Manitoba, Winnipeg, Canada, examines the pathogenic variability of L. maculans isolates from the Canadian Prairies and North Dakota each year. During 2002, the presence of PG3 (virulent on W and G and avirulent on Q) was reported in Manitoba (1). During 2003, a canola field located at La Riviere, Manitoba, 200 km southwest of Winnipeg, was found to be severely affected by blackleg. Stubble from this field was arbitrarily collected in mid-April 2004, and 98 single-pycnidia pure cultures were obtained by isolating fungi from surface-sterilized (2% sodium hypochlorite), infested residue, cultured on V8 agar at room temperature under cool-white florescent light for 24 h. Pycnidiospores were harvested after 14 days of incubation using the Miracloth filtering method (1). PG testing was performed using the three differential cultivars in the greenhouse. Known PG2, 3, and 4 isolates, 86-12, Liffole-6, and PL30.2, respectively, were included as positive controls. For each of the 98 isolates, 12 7-day-old cotyledons of each differential cultivar grown in Metro Mix were wound-inoculated with 10 μl of a pycnidiospore suspension (1 × 107 per ml) (1). Inoculated plants were maintained in the greenhouse (16/21°C night/day and a 16-h photoperiod with cool-white florescent light). The experiment was repeated three times. Disease severity on cotyledons was assessed 12 days after inoculation with a 0 to 9 scale (0 to 2 = resistant; 3 to 6 = intermediate; and 7 to 9 = susceptible). Of the 98 isolates tested, five were PG1, 51 were PG2, 24 were PG3, 13 were PGT, and five were PG4. The isolates classified as PG4 gave IP reactions of 7 to 9, 7 to 9, and 6.6 to 8.2, on W, G, and Q, respectively. PG3 was reported one year ago, but highly virulent isolates belonging to PG4 have not been previously detected in Manitoba. To our knowledge, this is the first report of the occurrence of PG4 isolates of L. maculans, and the first report of PG4 causing canola blackleg in Manitoba. The appearance of PG4 may be evidence of pathogen population changes occurring under high-selection-pressure exerted by resistance genes in commercial cultivars (2), or through importation of PG4 isolates with canola seed. References: (1) W. G. D. Fernando and Y. Chen. Plant Dis. 87:1268, 2003. (2) B. J. Howlett. Can. J. Plant Pathol. 26:245, 2004. (3) M. Keri et al. Can. J. Plant Pathol. 23:199, 2001. (4) A. Mengistu et al. Plant Dis. 75:1279, 1991.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.002 | 0.001 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".