Identification and molecular characterization of the blueberry stunt phytoplasma in Canada
Bibliographic record
Abstract
Blueberry farms in Quebec, Canada, were recently surveyed for phytoplasma-associated disease presence. Farmers previously reported a number of high bush blueberries showing stunt symptoms in three blueberry farms. Bushes were tested for phytoplasma based on the 16S rRNA gene. Nested PCR with universal phytoplasma primers resulted in amplification of phytoplasma DNA from blueberry bushes in samples from two of the farms surveyed. The 16S rDNA of the blueberry stunt phytoplasma from Quebec showed a 99% sequence identity with that of the blueberry stunt phytoplasma from Michigan (AY265220) from group 16SrI ‘Candidatus Phytoplasma asteris’ subgroup 16SrI-E. Phylogeny results confirmed the clustering of the blueberry stunt phytoplasma from Quebec within the same phylogenetic branch of phytoplasmas of group 16SrI, closely related to subgroup 16SrI-E. Preliminary virtual RFLP confirmed the sequence and phylogeny classification results. Further actual and virtual analyses are in progress to verify the presence of other possible phytoplasma strains from the Quebec blueberry farms.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".