Reactivation of <i>Giardia lamblia</i> cysts after exposure to low-pressure UV irradiation
Bibliographic record
Abstract
In this study, we determined the repair capabilities of Giardia lamblia cysts when they were exposed to low-pressure (LP) UV and then 4 different repair conditions. A UV collimated beam apparatus was used to expose shallow suspensions of G. lamblia cysts in buffered reagent water (PBS, pH 7.2) to various doses of LP UV irradiation. After UV irradiation, samples were exposed to 4 repair conditions (light and dark repair conditions with 2 temperatures (25 °C and 37 °C) for each condition). The inactivation of G. lamblia cysts by LP UV was very extensive (∼ 5 log10) even with a low dose of LP UV (1 mJ/cm(2)). More importantly, there was significant restoration of infectivity in G. lamblia cysts when they were exposed to a low dose of LP UV and then to all the repair conditions tested. Overall, the results of this study indicate that G. lamblia cysts do have the ability to repair their UV-damaged DNA when they are exposed to low doses of LP UV irradiation. This is the first study to report the presence of repair in UV-irradiated G. lamblia cysts.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".