Evaluation of Filters for the Sampling and Quantification of RNA Phage Aerosols
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Full frame distilled prediction
Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
- Candidate categories
- none
- Consensus categories
- none
- Domain
- Candidate signal: noneConsensus signal: none
- Study design
- Candidate signal: Bench or experimentalConsensus signal: Bench or experimental
- Genre
- Candidate signal: EmpiricalConsensus signal: Empirical
- Teacher disagreement score
- 0.067
- Threshold uncertainty score
- 0.570
- Validation status
machine_predicted_unvalidated·codex-gemma-dda1882f352a
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.002 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
- Teacher spread
- 0.279 · how far apart the two teachers sit on this one work
- Validation status
score_only:v0-immature-baseline· verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it
Abstract
This study exploits the virulent bacteriophages phi 6 (dsRNA) and MS2 (ssRNA) as surrogates for airborne RNA viruses.Two different filter types, polytetrafluoroethylene (PTFE) and polycarbonate (PC), were tested for their efficiency in collecting aerosolized RNA phages.Two commercial kits were tested for total RNA isolation.Also, heat shock treatments were performed in three different media to obtain the most favorable conditions for reverse transcription assays of dsRNA.Our findings suggest that PC filters are more suitable to recover infectious airborne RNA viruses as determined by plaque assays.Both types of filters were equally efficient in recovering RNA from aerosolized phage phi 6 as established by qRT-PCR.Viral samples should be treated with QIAamp Viral RNA Mini Kit and a 5 min heat shock treatment at 110 C in TE buffer before RT-PCR to maximize detection of phage phi 6.Overall, the infectivity of the recovered phages was severely affected by the aerosolization/air sampling process and the presence of RNA viruses in air samples should be determined by qRT-PCR.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- Aerosol Science and Technology
- Topic
- Bacteriophages and microbial interactions
- Field
- Environmental Science
- Canadian institutions
- Université LavalInstitut universitaire de cardiologie et de pneumologie de Québec
- Funders
- Natural Sciences and Engineering Research Council of Canada
- Keywords
- RNAAerosolizationRNA silencingVirologyInfectivityBacteriophageNucleic acidMicrobiologyChemistryBiologyMolecular biologyVirusRNA interferenceEscherichia coliBiochemistryGene
- Has abstract in OpenAlex
- yes