Capsaicin-sensitive sensory nerves, TRPV1 receptors and tachykinins play important roles in mast cell tryptase-induced arthritis and hyperalgesia
Bibliographic record
Abstract
Protease-activated receptors (PARs) are G protein-coupled receptors activated through proteolytic cleavage. They are localized on epithelial, endothelial and inflammatory cells, as well as on Transient Receptor Potential Vanilloid 1 (TRPV1) receptor-expressing capsaicin-sensitive sensory nerves. Tachykinins, such as substance P (SP) and neurokinin A (NKA) encoded by the TAC1 gene are released from these fibres and play an important role in inflammatory and nociceptive processes. We investigated the involvement of capsaicin-sensitive peptidergic afferents, TRPV1 ion channels and TAC1-encoded tachykinins in mast cell tryptase (MCT)-induced joint swelling, hyperalgesia and synovial microcirculation. The natural PAR2 activator MCT (20 µl, 12 µg/ml) was injected into the right tibiotarsal joint of mice. Pretreatment with high doses of the TRPV1 receptor agonist resiniferatoxin (RTX) was used to selectively inactivate capsaicin-sensitive peptidergic sensory nerves. TRPV1, TAC1 and neurokinin 1 receptor (NK 1 ) gene-deficient animals were also studied compared to their wild-type (WT) C57Bl/6 counterparts. Knee diameter was measured with a digital micrometer, mechanonociceptive threshold with dynamic plantar aesthesiometry and spontaneous weight distribution with incapacitance tester throughout a 6-hour period. Synovial bloodflow in urethane-anaesthetized animals was determined by laser Doppler imaging. In these studies, MCT was applied topically on the joints. MCT-induced joint swelling and secondary hyperalgesia were significantly reduced in TAC1 and NK 1 mice, but not in the other groups compared to WTs. Spontaneous weight distribution decreased by 10% on the injection site in response to MCT in WT mice, but not in any other groups. Synovial vasodilatation in response to topical MCT application was significantly smaller not only after the destruction of the capsaicin-sensitive afferents by RTX pretreatment, but also by the selective genetic deletion of the TRPV1 ion channels, but was not altered in TAC1 and NK 1 -deficient mice. These data provide evidence that MCT-evoked acute oedema and hyperalgesia are mediated by tachykinins through NK 1 receptor activation. The lack of difference observed in RTX-desensitized and TRPV1 mice is likely to be explained by a counteracting effect of simultaneously released inhibitory peptides (e.g. somatostatin, endomorphins) from the same capsaicin-sensitive fibres. In contrast, these afferents and the TRPV1 receptors are essential in acute synovial vasodilatation, but tachykinis are not involved in this response.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".