A TWO DIMENSIONAL GEL ELECTROPHORESIS TECHNIQUE COMBINING A MICRO‐ AND A MINI‐GEL SYSTEM FOR PROTEOMIC ANALYSIS OF FUNGAL HEAT SHOCK PROTEINS
Bibliographic record
Abstract
Abstract A two‐dimensional gel electrophoresis (2‐DGE) technique is described here that combines isoelectric focusing (IEF) based separation of proteins using the PhastGel IEF in the first dimension and the Criterion precast mini‐gels in the second dimension. The technique is rapid (2.5 to 3 h including silver staining), convenient, sensitive with high resolution and enables simultaneous analysis of up to 24 samples. This technique also eliminates any inconsistencies arising from gel to gel variation, either from electrophoresis running conditions or differential staining times, especially as in the case of silver stains. The rapidity and efficacy of this technique is demonstrated by the proteome‐based analysis of the induction, enhancement and repression of proteins in heat shocked Beauveria bassiana, ah entomopathogenic fungus.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".