Determination of isoelectric point and investigation of immunoreaction in peanut allergenic proteins–rabbit IgG antibody system by whole‐column imaged capillary isoelectric focusing
Bibliographic record
Abstract
Abstract The recently developed whole‐column imaged capillary isoelectric focusing is an advanced technique for the pI determination of proteins. In this study, we initially applied this technique to determine the pI of peanut allergens and its antibody, rabbit IgG. Two main proteins, Ara h I and Ara h II, were found in the peanut allergen solution. Their pI values were determined to be about 4.5–4.7 and 5.1–5.6, respectively. The value of pI for the antibody rabbit IgG was 6.1–6.5. Second, the immunoreaction selectivity of the two main peanut allergenic proteins with antibody rabbit IgG was studied. The CIEF results show that the peanut allergen Ara h II has a larger affinity for binding to the antibody rabbit IgG than Ara h I. © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 322–326, 2001
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".