Growth Hormone Receptor Binding Protein in Rat Anterior Pituitary
Bibliographic record
Abstract
GH is synthesized by the somatotrophs in the pituitary, where it may have paracrine actions. In order to identify the GH target cells in rat pituitary, the cellular distributions of rat GH receptor binding protein messenger ribonucleic acid (rGH-RBP mRNA) and protein were investigated at the electronmicroscopic level, using in situ hybridization and immunocytology, respectively, on ultrathin frozen sections of rat pituitary. Ultrastructural distribution of 125I-bGH, 30 min after intracardiac administration was also performed in order to determine the pituitary cell types that bind the labeled hormone. In situ hybridization was performed using digoxigenin-labeled oligonucleotide probe revealed by indirect immunogold reaction. rGH-RBP mRNA was readily identified in the cytoplasmic matrix, associated with the endoplasmic reticulum and the nucleus of the somatotrophs, the lactotrophs and the gonadotrophs. No significant signal was detected in the corticotrophs or thyrotrophs. The number of gold particles in each pituitary cell type was estimated by direct counting, and was compared to the results of hybridization performed on rat liver sections as a control. The results showed that the level of rGH-RBP mRNA was higher in hepatocytes than in the pituitary cells, and was higher in the somatotrophs and lactotrophs than in the gonadotrophs. Immunocytological detection of rGH-RBP was performed using two monoclonal antibodies (mAbs 43 and 263) directed against independent epitopes of the extracellular domain of the rGH-R. Indirect immunocytological detection showed regionalization of rGH-RBP; it was present in the cytoplasmic matrix and the nucleus of the hepatocytes and in discrete pituitary cells: somatotrophs, lactotrophs and gonadotrophs, but not in thyrotrophs or corticotrophs. Gold particle number was also higher in somatotrophs and lactotrophs than in gonadotrophs and higher in the nucleus compared to the cytoplasmic matrix. Radioiodinated GH was uptaken 30 min after injection by the same three pituitary cell types, showing evidence for the functional role of the GH receptor. In conclusion, we find that the cellular localization of rGH-RBP mRNA and protein is similar in discrete cell subpopulations of the pituitary, suggesting a direct effect of GH on somatotrophs, lactotrophs and gonadotrophs, through paracrine, autocrine or intracrine mechanisms.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".