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Record W2062693731 · doi:10.1117/12.797219

DNA-programmed protein-nanoelectronic transducer array

2008· article· en· W2062693731 on OpenAlexfundno aff
Gary Withey, Jin Ho Kim, Jimmy Xu

Bibliographic record

VenueProceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · 2008
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicAdvanced biosensing and bioanalysis techniques
Canadian institutionsnot available
FundersMcMaster University
KeywordsBiomoleculeScalabilityBiosensorTransducerNanotechnologyMultiplexingAnalyteOligonucleotideComputer scienceDNAMaterials scienceEngineeringChemistryElectrical engineering

Abstract

fetched live from OpenAlex

By incorporating DNA as addressable linkers, we can direct and coordinate the simultaneous, parallel self-assembling and binding of multiple different redox proteins to designated nanoelectrodes. As a result, we have formed a nanoelectronic-protein transducer array which is capable of real-time, multiplexed detection of several analytes in parallel. The sequence-specificity of DNA hybridization provides the means of encoding spatial address instruction to the otherwise random self-assembling process and enables the desired programmability, scalability, and renewability. Results of this study, under an AFOSR MURI program, demonstrate the feasibility of a new paradigm of biosensing: detection of not only the presence of target substances but also the real-time activities of multiple biomolecules. In this system, the conjugated biomolecules and nanoelectronic components provide the active monitoring and mediating functions in real time, and can be integrated en masse into large arrays in a silicon-based integrated circuit.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.229
Teacher spread0.221 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2008
Admission routes1
Has abstractyes

Explore more

Same venueProceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE→Same topicAdvanced biosensing and bioanalysis techniques→French-language works237,207→