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Record W2062924532 · doi:10.1038/nnano.2015.19

An endogenous nanomineral chaperones luminal antigen and peptidoglycan to intestinal immune cells

2015· article· en· W2062924532 on OpenAlexaff
Jonathan J. Powell, Emma Thomas-McKay, Vinay Thoree, Jack Robertson, Rachel E. Hewitt, Jeremy N. Skepper, Andy Brown, Juan Carlos Hernández‐Garrido, Paul A. Midgley, I. Gómez-Morilla, G.W. Grime, K.J. Kirkby, Neil A. Mabbott, David S. Donaldson, Ifor R. Williams, Daniel Ríos, Stephen E. Girardin, Carolin Haas, Sylvaine F. A. Bruggraber, Jon D. Laman, Yakup Tanriver, Giovanna Lombardi, Robert I. Lechler, R. P. H. Thompson

Bibliographic record

VenueNature Nanotechnology · 2015
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGut microbiota and health
Canadian institutionsCanada Research Chairs
FundersNational Institute of Diabetes and Digestive and Kidney DiseasesBiotechnology and Biological Sciences Research CouncilEngineering and Physical Sciences Research CouncilMedical Research CouncilNational Institutes of HealthSir Halley Stewart TrustNational Institute of Allergy and Infectious DiseasesScience and Technology Facilities CouncilEuropean CommissionAlexander and Margaret Stewart Trust
KeywordsImmune systemPeptidoglycanCell biologySmall intestineBiologySecretionAntigenIntestinal mucosaChemistryBiophysicsBiochemistryImmunologyEnzymeMedicine

Abstract

fetched live from OpenAlex

In humans and other mammals it is known that calcium and phosphate ions are secreted from the distal small intestine into the lumen. However, why this secretion occurs is unclear. Here, we show that the process leads to the formation of amorphous magnesium-substituted calcium phosphate nanoparticles that trap soluble macromolecules, such as bacterial peptidoglycan and orally fed protein antigens, in the lumen and transport them to immune cells of the intestinal tissue. The macromolecule-containing nanoparticles utilize epithelial M cells to enter Peyer's patches, small areas of the intestine concentrated with particle-scavenging immune cells. In wild-type mice, intestinal immune cells containing these naturally formed nanoparticles expressed the immune tolerance-associated molecule ‘programmed death-ligand 1’, whereas in NOD1/2 double knockout mice, which cannot recognize peptidoglycan, programmed death-ligand 1 was undetected. Our results explain a role for constitutively formed calcium phosphate nanoparticles in the gut lumen and show how this helps to shape intestinal immune homeostasis. Calcium phosphate nanoparticles formed naturally in the intestine aid the transport of soluble molecules from the gut lumen to immune cells of the intestinal tissue, and contribute to the immune surveillance and homeostasis of the gut.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.260
Teacher spread0.248 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations92
Published2015
Admission routes1
Has abstractno

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