Role of motAB in Adherence and Internalization in Polarized Caco-2 Cells and in Cecal Colonization of Campylobacter jejuni
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Bibliographic record
Abstract
Campylobacter jejuni, a gram-negative motile bacterium commonly found in the chicken gastrointestinal tract, is one of the leading causes of bacterial gastroenteritis in humans worldwide. An intact and functional flagellum is important for C. jejuni virulence and colonization. To understand the role of C. jejuni motility in adherence and internalization in polarized Caco-2 cells and in cecal colonization of chickens we constructed a C. jejuni NCTC11168 V1 ΔmotAB mutant. The motAB genes code for the flagellar motor, which enables the rotation of the flagellum. The nonmotile ΔmotAB mutant expressed a full-length flagellum, which allowed us to differentiate between the roles of full-length flagella and motility in the ability of C. jejuni to colonize. To study the adherence and invasion abilities of the C. jejuni ΔmotAB mutant we chose to use polarized Caco-2 cells, which are thought to be more representative of in vivo intestinal cell architecture and function. Although the C. jejuni ΔmotAB mutant adhered significantly better than the wild type to the Caco-2 cells, we observed a significant reduction in the ability to invade the cells. In this study we obtained evidence that the flagellar rotation triggers C. jejuni invasion into polarized Caco-2 cells and we believe that C. jejuni is propelled into the cell with a drill-like rotation. The ΔmotAB mutant was also tested for its colonization potential in a 1-day-old chicken model. The nonmotile C. jejuni ΔmotAB mutant was not able to colonize any birds at days 3 and 7, suggesting that motility is essential for C. jejuni colonization.Nota de Investigación—Papel del gene motAB en la adherencia e internalización de Campylobacter jejuni en células Caco-2 polarizadas y en su colonización cecal.Campylobacter jejuni, una bacteria gram negativa móvil que se encuentran comúnmente en el tracto gastrointestinal de pollo, es una de las principales causas de gastroenteritis bacteriana en los seres humanos en todo el mundo. Un flagelo intacto y funcional es importante para la virulencia y la colonización por C. jejuni. Para entender el papel de la motilidad de C. jejuni en la adherencia e internalización en células Caco-2 polarizadas y en la colonización cecal en pollos, se construyó una mutante de C. jejuni denominada NCTC11168 V1 ΔmotAB. Los genes motAB codifican para el motor flagelar, que permite la rotación del flagelo. La mutante no móvil ΔmotAB expresaba un flagelo completo, lo que permitió diferenciar entre las funciones de los flagelos completos y la movilidad en la capacidad de C. jejuni para colonizar. Para estudiar la capacidad de adherencia y la invasividad de la mutante de C. jejuni denominada ΔmotAB se optó por utilizar células Caco-2 polarizadas, que se cree que son más representativas de la arquitectura de las células intestinales y de su función in vivo. Aunque la mutante de C. jejuni ΔmotAB mostró una adherencia significativamente mejor a las células Caco-2 que la bacteria de tipo silvestre, se observó una reducción significativa en la capacidad de invadir las células. En este estudio se obtuvo evidencia de que la rotación flagelar desencadena la invasión por C. jejuni en células Caco-2 polarizadas y se cree que C. jejuni es propulsada hacia dentro de las células con una rotación con perforación. La mutante ΔmotAB también se analizó en su potencial de colonizar en un modelo de pollos de un día de edad. La mutante de C. jejuni no motil ΔmotAB no fue capaz de colonizar en ninguna de las aves a los días 3 y 7, lo que sugiere que la motilidad es esencial para la colonización C. jejuni.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it