Genetic and Morphological Comparisons of New and Old World Populations of <I>Spalangia</I> Species (Hymenoptera: Pteromalidae)
Bibliographic record
Abstract
The genetic similarity of New and Old World samples of Spalangia spp. (Hymenoptera: Pteromalidae) was examined using two ribosomal DNA regions. The species examined were Spalangia cameroni Perkins, Spalangia endius Walker, Spalangia gemina Bouc˘ek, Spalangia nigra Latreille, Spalangia nigroaenea Curtis, and Spalangia slovaca Bouc˘ek. Two species of Muscidifurax, Muscidifurax raptor Girault & Sanders and Muscidifurax zaraptor Kogan & Legner (Hymenoptera: Pteromalidae) were included as outgroup taxa. The internal transcribed spacer (ITS)-1 region was highly variable among Spalangia species with many insertions/deletions making alignment of the sequences difficult. The D2-D3 region of the 28s ribosomal gene and the nuclear rDNA 18s gene were more conserved and enabled phylogenetic analysis. No genetic differentiation was observed among S. cameroni and S. endius samples from Kazakhstan, Russia, and North America. New World samples of S. nigroaenea are genetically distinct from S. slovaca, a morphologically similar Old World species that is newly recorded from Kazakhstan and Russia. The intact 920 bp ITS-1 amplicon of S. nigroaenea was much larger than the 780-bp amplicon of S. slovaca. Kimura two-parameter genetic distance between the two species was 0.015 for the 28s region. Otherwise, the smallest genetic distance among recognized Spalangia species was 0.037 between S. endius and S. nigra. The genetic distance between M. raptor and M. zaraptor was 0.004. Based upon these results, the utility of the D2-D3 region of the 28s ribosomal gene is substantiated for differentiating species of Spalangia. The molecular analysis of the six Spalangia species revealed two groupings: S. nigroaenea and S. slovaca and S. cameroni and S. gemina. A third clade, S. endius and S. nigra, was observed, but bootstrap support was weak. These relationships were compared with those indicated by morphology and all agreed except possibly between S. endius and S. nigra, for which morphological evidence is equivocal. Morphological features are described and illustrated to distinguish the morphologically most similar species, S. nigra, S. nigroaenea, and S. slovaca, from each other and from other Spalangia spp.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".