Ultrastructural features of the acetylcholine innervation in the developing parietal cortex of rat
Bibliographic record
Abstract
To follow on a recent quantitative study of the developing cholinergic (ACh) innervation in rat neocortex, axon varicosities identified by electron microscopic immunocytochemistry with a highly sensitive antibody against choline acetyltransferase (ChAT) were examined in the primary somatosensory area (Par1) of rats at postnatal ages (days) P8, P16, and P32. As visualized and measured in single thin sections, and compared with those of unlabeled varicosities selected at random in the same photomicrographs, the ChAT-immunostained profiles displayed intrinsic and relational features very similar to those previously described in the same cortical area of adult rat (Umbriaco et al. [1994] J. Comp. Neurol. 348:351-373). At the three postnatal ages, the immunoreactive profiles were comparable in shape, size, and vesicular content in all cortical layers, but showed an increasing frequency of mitochondria with age, reaching 44% at P32. Synaptic junctions were observed on 6.3 to 8.7% of these sectional profiles, indicating an average synaptic incidence of 17% for whole varicosities, again comparable to that in the adult (14%). As in adult, the junctions made by the rare synaptic ChAT-immunostained varicosities were always single, usually symmetrical, and more frequently found on dendritic branches than spines. Thus, cortical ACh varicosities displayed intrinsic and relational features similar to adult ones as soon as this innervation was installed, suggesting that a diffuse mode of transmission and ambient level of ACh could play a major role in the diverse effects of this neuromodulator during cortical development.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".