Characterization and Prokaryotic expression of Glucuronyltransferase-S Gene in Silkworm Bombyx mori
Bibliographic record
Abstract
As genome of B. mori is available in GenBank, identification of novel genes of B. mori can be carried out. In this study,we used the in silico cloning method to obtain the Glucuronyltransferase-S (GlcAT-S) gene of B. mori and analysedwith bioinformatics tools. The result was confirmed by RT-PCR,prokaryotic expression and western blot. The GlcAT-ScDNA contains a 843bp ORF and has three exons. The deduced protein has 280 amino acid residues, with the predictedmolecular weight of 31842. 02 Da, isoelectric point of 9.16, and contains conserved GlcAT domains. The protein showshigh degrees of identity with that of some homologous protein from other species.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".