Pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase overexpression in transgenic tobacco: physiological and biochemical analysis of source and sink tissues
Bibliographic record
Abstract
Transgenic Nicotiana tabacum L. (tobacco) plants expressing an unregulated gene for pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase (PFP) from the fermentor protist Giardia lamblia were produced. Independently transformed lines revealed a high level of Giardia PFP activity but unaltered activities of native plant PFP, phosphofructokinase, and fructose-1,6-bisphosphatase. Transgenic plants exhibited a decrease in total biomass but no dramatic physiological or morphological alterations or significant reduction of seed yield. Both source and sink tissues demonstrated altered partitioning: leaf starch was significantly lower at the beginning and end of the daily light period and young seeds had lower starch but higher lipid levels, and these changes were correlated with PFP activity. Transgenic seeds had significantly higher percentages of germination, and microscopic examination of these seeds showed a temporal enhancement in growth of the young embryo. The significance of these results as they relate to our current understanding of PFP is discussed.Key words: pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase (PFP), transgenic, Nicotiana tabacum, glycolysis, photosynthate partitioning.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".