Detection of aster yellows phytoplasma DNA in seed and seedlings of canola (<i>Brassica napus</i>and<i>B. rapa</i>) and AY strain identification
Bibliographic record
Abstract
A study was conducted to identify the strains of aster yellows (AY) disease present in crops of Brassica napus and B. rapa grown near Medstead, Saskatchewan. AY phytoplasma DNA was detected in midrib, stem and root tissues of several symptomless plants as well as plants exhibiting typical AY disease symptoms. Most symptomatic and symptomless, AY-infected plants produced normal-looking and misshapen seeds. However, for both Brassica species, symptomatic plants produced significantly more seeds containing phytoplasma DNA than symptomless, AY-infected plants. Also, significantly more misshapen seeds contained phytoplasma DNA than normal seeds. Phytoplasma DNA belonging to subgroups 16SrI-A and 16SrI-B was detected in symptomatic and symptomless, AY-infected plants and in seed of these plants. The new AY strain sequences were registered in Genbank. The study reports for the first time the detection of AY strains in seedling tissues of both Brassica species. The research also showed that spiral cleaning has the potential to remove seeds that contain phytoplasma DNA in B. napus.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".