Culture of Rabbit Embryonic Germ Cells Derived from Primordial Germ Cells
Bibliographic record
Abstract
Abstract Zhang, R., Chang, W. and Han, J-Y. 2004. Culture of rabbit embryonic germ cells derived from primordial germ cells. J. Appl. Anim. Res., 26: 61–66. Primordial germ cells (PGCs) were developed as new material for isolation and cloning of embryonic stem (ES) cells, which were designated as embryonic germ cells (EG) to distinguish their tissue origin. The collected PGCs were cultured on feeder layers obtained from mitotically inactivated mouse or rabbit fibroblasts in Dulbecco's modified Eagle's medium supplemented with soluble recombinant human leukemia inhibitory factor (20 ng/ml), stem cell factor (30 ng/ml), forskolin (20 ng/ml). Two stable undifferentiated EG cell lines were established and were passed to fresh feeders every week for maintenance of their undifferentiated state. The established EG cell lines were pluripotent and were capable of forming embryonic bodies which differentiated into varied cell types including autorhythmic cells without efficient feeder layers. By comparison of growth behaviors of EG cells, we found that homogenous are superior to heterogenous fetal fibroblast feeder layers to support the EG growth.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".