C5a plays a role in PMN infiltration into the DSS inflamed colonic mucosa
Bibliographic record
Abstract
A role for complement in colitis has been implicated yet it is unclear whether it promotes or possibly inhibits inflammation. As C5a is a potent PMN chemoattractant and PMN infiltrate the mucosa during dextran sulphate sodium (DSS) colitis, we hypothesized that C5aR−/− mice would experience less colitis due to reduced PMN infiltration. Balb/c and C5aR−/− mice were fed 5% DSS in their drinking water for 5 days then were euthanised on day 6 and their colons examined. Balb/c mice experienced limited clinical illness. Their mid‐colons were inflamed with PMN infiltrates in the mucosa, crypt loss, and small focal ulcers. In contrast, C5aR−/− mice experienced a more severe colitis, with greater ulcers, but interestingly PMN remained primarily in the submucosa. Staining for F4/80 indicated that a substantially greater number of macrophages infiltrated the C5aR−/− colon versus the Balb/c mice. In order to rule‐out the possibility that PMN were damaging the colon from the submucosa, C5aR−/− mice receiving DSS were made neutropenic using injections of Gr‐1 antibody. Despite abolishing PMN infiltration, these mice still demonstrated high numbers of mucosal F4/80+ cells and colon damage. Thus while C5a does play a role in PMN recruitment into the mucosa, our hypothesis was incorrect and C5aR−/− mice in fact experience heightened colitis, with macrophages possibly mediating the inflammation. Supported by the CCFC.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".