Quercetin-3-rutinoside, a flavonol glycoside from Teucrium polium suppresses advanced glycation end products (AGEs) formation: A structural study
Bibliographic record
Abstract
In this study, isolation and structure elucidation of the high antioxidant and antiglycation compound(s) from Teucrium polium were performed. Based on our results, rutin (quercetin-3-rutinoside ) , a flavonol glycoside isolated from T. polium exhibited high antioxidant activity compared to the other isolated compounds from T. polium. The protein glycation inhibitory activity of rutin was also evaluated in vivo using various models [1, 2, 3]. In the early stage of protein glycation rutin showed a moderately inhibitory activity on HbA 1C formation, which were similar to that of aminoguanidine, a well-known inhibitor for advanced glycation endproducts (AGEs). For the middle stage, rutin developed more significant inhibitory effect on methylglyoxal-medicated protein modification, and in the last stage of glycation, rutin was found to be potent inhibitor of both the AGEs formation and the subsequent cross-linking of proteins. Furthermore, the effect of rutin on preventing oxidative protein damages including effect on protein carbonyl (PCO) formation and thiol oxidation which are believed to form under the glycoxidation process was achieved. Rutin inhibited high glucose induced oxidative damages to protein by decreasing PCO formation and preventing thiols group from oxidation. In addition, the structural changes of human serum albumin with glucose, in the presence of rutin were evaluated by circular dichroism and fluorescence techniques. Regarding enhancing the helicity of the protein and prevents helix decrement in the secondary structure of human serum albumin in the presence of glucose, it can be concluded that rutin may be act as an anti-glycation agent for human serum albumin. Acknowledgements: This research work was supported by the Research Council of Shahid Beheshti University (G.C), Tehran, Iran. We also extend our thanks to Mrs. M. Ashorzadeh for her instrumental assistance. References: [1]Rahbar, S. et al. (2000) Mol. Cell Biol. Res. Commun. 3:360–366. [2] Lee, C. et al. (1998)J. Biol. Chem. 273:25272–25278. [3] Nagarai, R.H. et al. (1996)J. Biol. Chem. 271:19338–19345.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".