Cellular expression of the pepsinogen and gastric proton pump genes in the stomach of winter flounder as determined by <i>in situ</i> hybridization
Bibliographic record
Abstract
The sites of pepsinogen synthesis and hydrochloric acid secretion were localized in the glandular stomach mucosa of winter flounder using in situ hybridization with gastric‐specific, biotinylated antisense RNA probes for three genes: pepsinogen IIa, pepsinogen IIb and the a‐subunit of the proton pump (H+/K+‐ATPase). The spatial expression patterns of the pepsinogen IIa and IIb genes appeared to be the same, but they differed from that of the ‐proton pump gene. The gastric gland cells contained transcripts (mRNAs) of all three genes, whereas the mucous neck cells and surface mucous cells contained transcript of the a‐proton pump only. The expression of the pepsinogen IIa, pepsinogen IIb and α‐proton pump gene in the same gastric gland cells indicates that these cells synthesize pepsinogens and secrete hydrochloric acid simultaneously and confirms that they are of the oxynticopeptic type. The presence of the a‐proton pump mRNA in the mucous neck and surface mucous cells, shown here for the first time, indicates that these cells are capable of secreting hydrochloric acid. The physiological importance of hydrochloric acid secretion by these two cell types remains unknown.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".