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Record W2074090908 · doi:10.1159/000205853

Reduction and Spectroscopic Properties of Hemoglobins M

2009· article· en· W2074090908 on OpenAlexaboutno aff
M. Nagai, S. Takama, Yosuke Yoneyama

Bibliographic record

VenueActa Haematologica · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicHemoglobin structure and function
Canadian institutionsnot available
Fundersnot available
KeywordsDithioniteFerredoxinSodium dithioniteChemistryReductaseFlavin groupFerredoxin—NADP(+) reductaseHistidineAbsorption (acoustics)EnzymeHemeAbsorption spectroscopyCrystallographyStereochemistryBiochemistryMaterials scienceInorganic chemistry

Abstract

fetched live from OpenAlex

Reduction of five hemoglobins M (Hbs M) was studied using three enzymatic reducing systems; namely NADPH-flavin reductase from human erythrocytes, NADH-cytochrome b5 reductase from human erythrocytes and ferredoxin-NADP reductase from spinach. Under anaerobic conditions, abnormal chains in Hb M Saskatoon were reduced by all three systems, whereas those in Hb M Milwaukee were reduced by the latter two enzyme systems only. The abnormal chains in Hb M Hyde Park were reduced only by the ferredoxin-NADP reductase system. On the other hand, the abnormal chains in Hb M Iwate and Hb M Boston were reduced by sodium dithionite, but not by the three enzymatic reducing systems. Absorption and circular dichroic (CD) spectra of these Hbs M were compared with those of Hb A to examine their structural abnormalities. Absorption and CD spectra of four Hbs M except Hb M Milwaukee were remarkably different from those of Hb A. Conformational differences between alpha- and beta-abnormal Hbs M were reflected in the CD spectrum of the ultraviolet region. The difference between the proximal and distal histidine-replaced Hbs M was also observed in the CD spectra of the visible region.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.224
Teacher spread0.213 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations11
Published2009
Admission routes1
Has abstractyes

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