Purification, crystallization and preliminary X-ray analysis of an unusual thioredoxin from the gastric pathogen<i>Helicobacter pylori</i>
Bibliographic record
Abstract
Thioredoxin-2 (HP1458) from Helicobacter pylori is a member of the thioredoxin family, but possesses the unusual active-site motif CPDC (compared with CGPC in other thioredoxins). H. pylori is deficient in the glutaredoxin system, making the thioredoxin system the sole reduction system in the bacterium and critical for its ability to survive oxidative stress. The recombinant protein has been overexpressed, purified and crystallized. This is the first reported crystallization of a thioredoxin possessing this unusual active site. Single crystals have been obtained using the sitting-drop technique. Crystals diffract to 2.4 A resolution and belong to space group P4(1), with unit-cell parameters a = b = 40.21, c = 64.65 A. Molecular replacement using AMoRe proved unsuccessful; however, implementation of the program BEAST gave successful molecular-replacement solutions.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.005 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".