Intracellular Localization of hsp90 Is Influenced by Developmental Stage and Environmental Estrogens in Rainbow Trout<i>Oncorhynchus mykiss</i>
Bibliographic record
Abstract
In this study, we investigated the intracellular localization of heat shock proteins hsp90 and hsp70 in adult and juvenile rainbow trout (Oncorhynchus mykiss) and in juvenile trout exposed to estrogen or one of its mimics, 4-nonylphenol (4-NP). Livers were harvested from each group and analyzed directly or separated into nuclear and nonnuclear fractions. We found that hsp70 was predominantly nonnuclear in mature and juvenile fish regardless of treatment. Mature fish had significantly greater levels of hsp90 outside the nucleus, while juvenile fish had similar levels of hsp90 inside and outside the nucleus. Treatment with estradiol or 4-NP resulted in a translocation of hsp90 out of the nucleus in juvenile fish. To our knowledge, this is the first study to demonstrate a development- and/or estrogen-dependent shift in intracellular localization of hsp90 in fish. This change in subcellular distribution points to important roles for this hsp in fish estrogen signaling and development.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".