Amplification and Molecular Characterization of DREB1A Transcription Factor Fragment From Finger Millet [(Eleusine coracana (L.) Gaertn]
Bibliographic record
Abstract
Studies have shown that several plant species posses DREB1A and DREB2A (Dehydration-Responsive Element Binding Protein) orthologs. DREB transcription factors, also called C-repeat binding factors (CBFs), are the transacting elements/ transcription factors first identified in Arabidopsis which bind to low-temperature and dehydration responsive element (LTRE/DRE) found in several dehydrin (Dhn) promoters as well as in promoters of other cold and drought responsive genes and involved in dehydration-, cold-, and salinity-regulated gene expression. In this study, a fragment of DREB1A ortholog named EcDREB1A has been amplified from finger millet (Eleusine coracana), an important drought-tolerant grain crop with a rich genetic diversity grown in semi-arid tropics. In the current study, a systematic approach has been taken to predict a theoretical primer for EcDREB1A based on the cloned Arabidopsis thaliana AtDREB1A and other DREB genes for orthologous gene identification from finger millet. Sixteen different but related nucleotide sequences based on AtDREB1A gene were retrieved from different databases. A highly conserved region of 287 bp was detected on multiple sequence alignments through claustalw2 program and a set of primers (forward and reverse) was predicted using Primer3plus and Net software on the basis of this conserved region, assuming ideal conditions for primer length, GC content, formation of primer-dimers, hairpin-loops etc. The amplified genomic fragment of EcDREB1A was found to be 536 bp long, with possible introns as per translational analysis. Longest detected ORF in the amplified EcDREB1A fragment encodes a putative protein of 84 amino acids rich in serine (13.10%) with a predicted molecular mass of 9.29 kDa. Multiple sequence alignment of this EcDREB1A fragment with other DREB genes revealed presence of 9 highly conserved amino acids. Allele mining of EcDREB1A gene fragment across selected 5 finger millet cultivars revealed no variations on nucleotide, probably due to narrow genetic base in the test materials. Identification of novel regulatory genes involved in abiotic stress tolerance and allele mining in a manner similar to this presented herein might lead to a better and quicker solution for improving stress tolerance in crop plants.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".