Cytosolic acidification and γ-aminobutyric acid synthesis during the oxidative burst in isolated<i>Asparagus sprengeri</i>mesophyll cells
Bibliographic record
Abstract
The four carbon, non-protein amino acid γ-aminobutyrate (GABA) accumulates rapidly in response to diverse stresses. Its synthesis is stimulated by increases in intracellular Ca 2+ or H + levels. The pathogen-induced oxidative burst is also associated with increases in Ca 2+ and H + levels. This study investigated the relationship between GABA synthesis and the oxidative burst. A Mas-7-induced consumption of oxygen in isolated Asparagus sprengeri Regel mesophyll cells was accompanied by rapid GABA synthesis. At pH 5.0, a 300% increase occurred within 16 min from 6.6 to 26.3 nmol GABA·10 6 cells 1 . At pH 6.0, the increase was from 8.5 to 18.1 nmol GABA·10 6 cells 1 . Mas-7 also stimulated rapid external alkalinization and intracellular acidification. Intracellular pH decreased 0.44 pH units at pH 5.0, and 0.21 pH units at pH 6.0. The Mas-7-induced oxidative burst, GABA synthesis, extracellular alkalinization, and intracellular acidification were all eliminated when lanthanum, a Ca 2+ channel blocker, replaced Ca 2+ in the incubation medium. The data demonstrate that GABA accumulation is associated with the oxidative burst, and results from the fluxes of H + and Ca 2+ , which are known to accompany the oxidative burst. They are discussed in light of emerging data that indicate a role for GABA in plant cell to cell signaling.Key words: γ-aminobutyric acid, GABA, oxidative burst.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".