Raman microspectroscopic evidence that dry‐fixing preserves the temporal pattern of non‐specific differentiation in live human embryonic stem cells
Bibliographic record
Abstract
Abstract Raman microspectroscopy allows the classification of populations of human embryonic stem cells (hESCs) in different stages of differentiation based on the relative intensities of certain amino acid and nucleic acid bands. Here, we report the results of a comparative study of the Raman spectra of live cells versus cells killed and fixed by rapid desiccation, focusing on the ratio of intensities at 757 cm −1 (tryptophan) and 784 cm −1 (DNA and RNA). We observe that the same temporal pattern emerges over a 3‐week time course in both sample types. This suggests that prolonged observations of dry‐fixed cells can yield high signal‐to‐noise chemical images that cannot be obtained from colonies of living cells where the time scale of significant biological changes are comparable to the time scale of the measurement. This permits, for example, comparison of the spatial distributions of cells at different stages of differentiation within the same colonies. Copyright © 2010 John Wiley & Sons, Ltd.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".