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PROLONGED EXERCISE AND NA+-K+-ATPASE ACTIVITY IN RAT SKELETAL MUSCLE

2001· article· en· W2082200089 on OpenAlexaff
Jonathon R. Fowles, D. J. Barr, Todd A. Duhamel, S Sandiford, Jonathan D. Schertzer, H Green

Bibliographic record

VenueMedicine & Science in Sports & Exercise · 2001
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMuscle metabolism and nutrition
Canadian institutionsUniversity of Waterloo
Fundersnot available
KeywordsInternal medicineEndocrinologyTreadmillVastus lateralis muscleChemistrySkeletal muscleATPaseEnzyme assayEnzymeMedicineBiochemistry

Abstract

fetched live from OpenAlex

The purpose of this study was to investigate the hypothesis that an acute bout of prolonged running exercise would result in a depression of maximal Na+-K+-ATPase activity in rat locomotor muscles, an effect that would be reversed during active recovery. Female Sprague Dawley rats (291 ± 9.8 gm, ± SE, n = 15), either ran on a treadmill (RUN) at 21m/min and 8% grade until fatigue or to a maximum of 2 h, acted as control (CON) or exercised for an additional 45 min at 10 m/min and % grade (REC). Tissue obtained from the soleus (SOL), red (RV) and white (WV) vastus lateralis was extracted and measured for Na+-K+-ATPase activity using the artificial substrate, 3-0-methylfluorescein (3-0MF). Although differences in 3-0MF were observed between tissue types (SOL > RV > WV; P < 0.05), the 1.7, 15.0 and 9.1% reductions observed for SOL, RV and WV, respectively in RUN were not significant (P > 0.05). Similarly not differences (P > 0.05) were observed between CON and REC regardless of tissue type. Values ± SE (μmol/h/mg protein) It is concluded that although the low intensity aerobic exercise induced a trend towards reductions in Na+-K+-ATPase activity, indicating possible structural alterations in the enzyme, the effects were not significant regardless of the fiber type characteristics of the locomotor muscles examined.Table

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.260
Teacher spread0.250 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2001
Admission routes1
Has abstractyes

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