Molecular and pathological characterization of N:O isolates of the<i>Potato virus Y</i>from Manitoba, Canada
Bibliographic record
Abstract
Previous studies have identified a recombinant isolate group of the Potato virus Y (PVY), PVYN:O, which exhibits a PVYO serotype and a PVYN pathotype. Molecular fingerprinting indicated that the isolates possess a European (Eu)–PVYN/NTN-like P1 gene and one PVYN-PVYO recombinant joint at the HC/Pro-P3 site. In this study, the complete genomic RNA sequence of two isolates of PVYN:O was determined. The overall identities at the polyprotein level between PVYN:O and other isolates ranged from 94.1% to 97.2%. However, the identities varied from 70.9% to 100% when individual mature proteins were compared. Scanning the nucleotide sequences revealed that PVYN:O comprises a recombinant genome in which the segment of nucleotides 1 to ~2400 is from Eu-PVYN, and the remainder from PVYO. Analysis of a population of PVYN:O isolates indicated the existence of pathological variants that induced different symptoms level in tobacco plants: severe, 16.7%; intermediate, 66.7%; and mild, 16.7%. Three representative isolates were selected for further analysis. Although pathologically different, the isolates were indistinguishable serotypically and genotypically when profiled using enzyme-linked immunosorbent assays and reverse transcription – polymerase chain reaction, respectively. No significant differences in nucleotide sequence were observed among the three isolates in 5'-UTR-P1-HC/Pro as well as the CP-3'-UTR region
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.002 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".