Molecular and pathological characterization of N:O isolates of the<i>Potato virus Y</i>from Manitoba, Canada
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Bibliographic record
Abstract
Previous studies have identified a recombinant isolate group of the Potato virus Y (PVY), PVYN:O, which exhibits a PVYO serotype and a PVYN pathotype. Molecular fingerprinting indicated that the isolates possess a European (Eu)–PVYN/NTN-like P1 gene and one PVYN-PVYO recombinant joint at the HC/Pro-P3 site. In this study, the complete genomic RNA sequence of two isolates of PVYN:O was determined. The overall identities at the polyprotein level between PVYN:O and other isolates ranged from 94.1% to 97.2%. However, the identities varied from 70.9% to 100% when individual mature proteins were compared. Scanning the nucleotide sequences revealed that PVYN:O comprises a recombinant genome in which the segment of nucleotides 1 to ~2400 is from Eu-PVYN, and the remainder from PVYO. Analysis of a population of PVYN:O isolates indicated the existence of pathological variants that induced different symptoms level in tobacco plants: severe, 16.7%; intermediate, 66.7%; and mild, 16.7%. Three representative isolates were selected for further analysis. Although pathologically different, the isolates were indistinguishable serotypically and genotypically when profiled using enzyme-linked immunosorbent assays and reverse transcription – polymerase chain reaction, respectively. No significant differences in nucleotide sequence were observed among the three isolates in 5'-UTR-P1-HC/Pro as well as the CP-3'-UTR region
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it