Waveguide evanescent field fluorescence microscopy: from cell-substratum distances to kinetic cell behaviour
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
We demonstrate an alternative to total internal reflection fluorescence (TIRF) microscopy. A method for imaging ultra thin films and living cells located on waveguides illuminated with their evanescent fields is introduced. Analysis of ion-exchanged waveguides focusing on their application as substrates for microscopic study of interfacial phenomena is presented. Various LB film stacks were imaged to verify the intensity interpretation due to the exponentially decaying evanescent fields of the waveguides. The paper gives an overview on the imaging applications of this technique. The fluorescence intensity has been used to determine quantitatively the cell attachment of osteoblasts (bone forming cells) to substrate surfaces. In live cell studies trypsin (a protease) was used to alter attachment of the cells to the substrate, as a means to demonstrate feasibility of the method in measuring attachment dynamics of cells in real time.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it