Electrochemical Detection of Hybridization of DNA Oligomers of Mixed Base Sequence by Surface-Stabilized Bilayer Lipid Membranes
Bibliographic record
Abstract
Surface stabilized bilayer lipid membranes (s-BLMs) made from egg phosphatidylcholine on silver wires have been used as transducers to electrochemically detect hybridization of short DNA oligomers. The ion currents through s-BLMs were used to monitor hybridization of 20 mer and 25 mer oligonucleotides. Single stranded DNA oligonucleotides that were modified by attachment of a 16 carbon aliphatic chain were immobilized onto the lipid membranes. Various quantities of complementary and partially complementary sequences (each without the aliphatic chain), were introduced as titrants to observe the degree of interaction with the immobilized DNA. The results indicated that hybridization could be detected for mixed base sequences, and for partially complementary sequences. Quantitative results are dependent on the degree of surface occupancy by DNA, on the degree of complementarity of sequences, and on the base sequence within the oligonucleotides.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".