Differential and systemic alteration of defence-related gene transcript levels in mycorrhizal bean plants infected with <i>Rhizoctonia solani</i>
Bibliographic record
Abstract
The role of arbuscular mycorrhizas in response of plants to soilborne root pathogens is unclear. A time course study was conducted to monitor disease development and expression of mRNA for the defence-related genes phenylalanine ammonia lyase, chalcone synthase, chalcone isomerase, and hydroxyproline-rich glycoprotein in bean (Phasoelus vulgaris L.) plants colonized by the arbuscular mycorrhizal fungus Glomus intraradices Schenck & Smith and postinfected with the soilborne pathogen Rhizoctonia solani Kühn. Precolonization of bean plants by G. intraradices did not significantly reduce the severity of rot symptoms. RNA blot analysis of the defence-related genes revealed a systemic increase in the four defence genes in response to R. solani infections. On the other hand, precolonization of bean plants with G. intraradices elicited no change in phenylalanine ammonia lyase, chalcone synthase, and chalcone isomerase transcripts. A differential and systemic alteration in the expression of all four defence genes was observed in all tissues only during the pathogenic interaction of arbuscular mycorrhizal beans. Depending on the time after infection with R. solani and the tissue examined, varying responses from stimulation to suppression to no change in transcript levels were detected.Key words: induced resistance, defence-related genes, RNA analysis, Rhizoctonia solani, Glomus intraradices.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".